TABLE 1.
Genotypic and phenotypic properties of STEC isolates from the calf cohort
| PFGE patterna | No. of isolates (n = 65) | Virulence gene pattern (PCR)
|
Adherence pattern and intensityb,c
|
|||
|---|---|---|---|---|---|---|
| stx type(s)/subtype(s) | eae subtypeb | Presence of ehxA | HEp2 | Bovine colonic epithelial | ||
| I | 14 | stx1, stx2 | eaezeta | + | LA, ++ | LA, + |
| II | 12 | stx1 | eaebeta1 | + | LA, +++ | LA, + |
| III | 10 | stx2, stx2c | eaebeta1 | + | LA, ++ | AA, + |
| IV | 4 | stx1 | eaeepsilon1 | + | LA, +++ | LA-AA, +++ |
| V | 4 | stx1 | eaezeta | + | LA, + | LA-AA, ++ |
| VI | 5 | stx2c | NT | NT | ||
| VII | 7 | stx2c | + | AA, +++ | NT | |
| VIII | 2 | stx2c | LA, + | LA-AA, + | ||
| IX | 1 | stx1 | eaeepsilon1 | + | NT | NT |
| X | 1 | stx1 | eaeepsilon1 | + | NT | NT |
| NA | 1 | stx2c | + | NT | NT | |
| NA | 1 | stx2c | NT | NT | ||
| NA | 1 | stx2, stx2c | NT | NT | ||
| NA | 1 | stx2c | + | NT | NT | |
| NA | 1 | stx2d | NT | NT | ||
a
NA, single isolates could not be assigned to a given PFGE pattern but were unique in their virulence gene pattern and therefore are listed as independent isolates.
b
One representative strain per PFGE pattern was used to determine the intimin serotype and the adherence pattern.
c
AA, aggregative; LA, local; LA-AA, mixed pattern; +, weak; ++, intermediate; +++, strong; NT, not tested.
d
Not subtypeable.