TABLE 2.
Stx1-specific antibodies in sera of dams and calves, detected by immunoblotting
| Calf/dam no. | Time of sampling | Stx1Ab titer (VNA)a | Isotype/subunit specificity of antibodies (immunoblotting)b
|
||
|---|---|---|---|---|---|
| IgG1 | IgG2 | IgA | |||
| Calf 21 | 1st wk | 433 | A++, B++ | A++ | A+ |
| 52nd wk | 1,706 | A+++ | A++, B+ | − | |
| Dam 21 | 594 | A++, B+ | A++, B+ | A+ | |
| Calf 17 | 24 h | 891 | A+++ | A++ | A++, B++ |
| 48th wk | 73 | A+++ | A++ | A+ | |
| Dam 17 | 293 | A++ | A++ | − | |
| Calf 2 | 24 h | 1,590 | A+++, B+ | A+ | A+++, B+ |
| 24th wk | − | A++ | A++ | A+ | |
| Dam 2 | 367 | A++, B+ | A+++ | A+ | |
| Calf 1 | 1st wk | 2,240 | A+++, B++ | A++ | A+ |
| 19th wk | 154 | A++ | A++ | − | |
| Dam 1 | 1,362 | A+++, B++ | A++ | − | |
| Calf 26 | p.n. | − | − | − | − |
| 24 h | − | A+ | A+ | A+ | |
| 40th wk | − | A++ | A++ | A+ | |
| Dam 26 | 150 | A++ | A++ | A+ | |
| Calf 20 | 24 h | 118 | A++ | A++ | A++ |
| 20th wk | 2,004 | A++ | A++ | A+ | |
| Dam 20 | 137 | A++ | A++ | A+ | |
| Calf 8 | 24 h | 2,163 | A++ | A++ | A++ |
| 24th wk | − | A++ | A++ | − | |
| Dam 8 | 2,986 | A++ | A++ | − | |
a
Titers below the detection limit of the VNA (titers of <30) are indicated as negative.
b
A, StxA1; B, StxB1; −, nil; +, weak; ++, intermediate; +++, strong.