TABLE 4.
Conserved amino acid residues of reducing clade III PKSs specific for different groups of fungi
| Domain | Relative positionsa | Conserved domain in the alignmentb | Conserved residues
|
|||
|---|---|---|---|---|---|---|
| All insect-pathogenic fungi | Insect biocontrol fungic | Ophiocordyceps genus | Wood-inhabiting fungi | |||
| KS | 65/65 | a | E(Q)KA | KH(T)P | ||
| 74/74 | b | LDE(D) | FKE(A) | |||
| 116/116 | c | LS | FT | |||
| Interdomain | 132/129/132 | d | V(I)VPR | -HK(R) | PTRK | |
| 139/139 | e | SGNDEGAAKRNATAI | TANDKASLEAVLKNL | |||
| 159/159 | f | H | R | |||
| 167/167 | g | RIIR | ALMA(S) | |||
| 178/178 | h | ERRT(S)H | QRRSL | |||
| 183/179/183 | i | HS(N)Y | L(M)PW | LQW | ||
| 199/199 | j | STLNSEAM | EAINGQR(K)L(P) | |||
a
Relative position using the residue number of the first member of group in the multiple alignment: IsjF1R1C4 (all insect-pathogenic fungi and insect biocontrol fungi), Opu2F1R2_D9 (Ophiocordyceps genus), and PyrF1R2B1 (wood-inhabiting fungi).
b
Conserved domains are shown in the multiple-sequence alignment in Fig. S2 in the supplemental material.
c
The insect biocontrol fungal group includes B. bassina, C. pseudomilitaris, I. javanica, M. flavoviride, and P. tenuipes.