TABLE 1.
Characteristics of S. cerevisiae strains and plasmids used in this study
| Strain/plasmid | Relevant genotype | Source and/or reference |
|---|---|---|
| S. cerevisiae strains | ||
| Type II | Bakery yeast, MATa/α | Sigma-Aldrich |
| IFO 0224 | Alcohol-fermenting yeast, MATa/α | National Institute of Technology and Evaluation |
| S3 | Shochu yeast no. 3, MATa/α | Brewing Society of Japan |
| FERM I-6 | Alcohol-fermenting yeast, MATa/α | Advanced Industrial Science and Technology |
| W1 | Wine yeast no. 1, MATa/α | Brewing Society of Japan |
| FERM I-7 | Alcohol-fermenting yeast, MATa/α | Advanced Industrial Science and Technology |
| W4 | Wine yeast no. 4, MATa/α | Brewing Society of Japan |
| S2 | Shochu yeast no. 2, MATa/α | Brewing Society of Japan |
| IR-2 | Alcohol-fermenting flocculent yeast, MATa/α | Advanced Industrial Science and Technology; 13 |
| MA-R1 | IR-2, AUR1 | 15 |
| MA-R4 | IR-2, AUR1::AUR1-C-[PGKp-XK-PGKt, PGKp-XDH-PGKt, PGKp-XR-PGKt] | 15 |
| MA-R5 | IR-2, AUR1::AUR1-C-[PGKp-XK-PGKt, PGKp-XDH(ARSdR)-PGKt, PGKp-XR-PGKt] | This study |
| Plasmids | ||
| pAUR101 | AUR1 (control plasmid) | Takara Bio |
| pAUR-XKXDHXR | AUR-C expression of XK, wild-type XDH, and XR genes | 17 |
| pAUR-XKARSdRXR | AUR-C expression of XK, mutated XDH, and XR genes | 17 |