TABLE 1.
Repeatability of v-PCR method
| Sample | Viable L. pneumophilaa
|
Heat-killed L. pneumophilab
|
||
|---|---|---|---|---|
| log10 GU/ml | Signal reduction (log units) | log10 GU/ml | Signal reduction (log units) | |
| Untreated control | 6.5 | 6.31 | ||
| EMA-treated replicate | ||||
| 1 | 6.32 | 0.18 | 2.97 | 3.34 |
| 2 | 6.3 | 0.2 | 2.74 | 3.58 |
| 3 | 6.26 | 0.24 | 3.17 | 3.14 |
| 4 | 6.34 | 0.16 | 3.01 | 3.31 |
| 5 | 6.34 | 0.16 | 2.89 | 3.43 |
a
The mean signal reduction for viable cells was 0.19 log units. The standard deviation of the reduction signal (expressed in log GU/ml) was 0.03.
b
The mean signal reduction for heat-killed cells was 3.36 log units. The standard deviation of the reduction signal (expressed in log GU/ml) was 0.16.