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. 1999 May 25;96(11):6285–6290. doi: 10.1073/pnas.96.11.6285

Table 1.

Kinetic characterization of the enzymatic activities with oxalacetate and pyruvate of T. vaginalis cell extract and genes expressed in E. coli

Enzyme sample Activity
Oxalacetate
Pyruvate
pH Km* Vmax pH Km* Vmax
Homogenate 7.6 67 23 7.2 175 5
TvLDH1 5.9 40 31 6.1 45 400
Leu91Arg 6.3 425 1,430 6.1 85 123
TvMDH 7.6 33 3,076 ND ND ND
Arg91Leu 6.5 222 400 ND ND ND

ND, not detectable. 

*

μM. 

μmol⋅min−1⋅mg protein−1