TABLE 2.

Bacteria isolated from DMSP, DMS, and acrylic acid enrichment cultures of mucus, tissue, and skeletal samples of the corals, A. millepora and M. aequituberculata, and PCR detection of genes homologous to dddR and dddD previously implicated in DMSP degradation

Enrichmenta	Closest taxonomically related strain(s)b	Strain sourcec	Taxonomic description	Alignment (bp)	Sequence identity (%)	Gene(s) involved in DMSP degradation
DMSP	Arhodomonas sp. strain EL-201	Acropora mucus	Gammaproteobacteria	682/699	97	dddR
	Spongiobacter nickelotolerans	Acropora tissues	Gammaproteobacteria	667/699	95	Other pathway
	Pseudomonas sp. strain CJ11075	Montipora tissues	Gammaproteobacteria	666/670	99	dddR, dddD, dddL
	Idiomarina sp. strain PR53-12	Acropora tissues and skeleton	Gammaproteobacteria	666/670	99	Other pathway
	Alteromonas sp. strain S1613	Acropora mucus and skeleton, Montipora tissues	Gammaproteobacteria	700/700	99-100	Other pathway
	Roseobacter sp. strain SOEmb11	Acropora and Montipora tissues	Alphaproteobacteria	685/700	98	dddR, dddD
DMS	Alteromonas sp. strain S1613 and CF6-3	Acropora mucus and skeleton, Montipora mucus	Gammaproteobacteria	698/699	99-100
	Pseudoalteromonas ruthenica	Acropora tissues and skeleton	Gammaproteobacteria	695/700	99
	Vibrio tubiashi	Acropora tissues	Gammaproteobacteria	699/700	99
	Vibrio sp. strains 6G8 and 1G4	Montipora tissues, Acropora tissues and skeleton	Gammaproteobacteria	700/700	100
	Photobacterium sp. strain 3F8	Montipora tissues	Gammaproteobacteria	698/699	98
	Idiomarina sp. strain JL110-118	Acropora skeleton	Gammaproteobacteria	700/700	100
Acrylic acid	Vibrio harveyi	Acropora mucus, Acropora tissues	Gammaproteobacteria	700/700	100
	Vibrio fischeri	Acropora tissues	Gammaproteobacteria	700/700	100
	Vibrio fortis	Acropora mucus, Acropora tissues	Gammaproteobacteria	641/642	89-98
	Photobacterium sp. strain 3F8	Acropora tissues	Gammaproteobacteria	694/695	99
	Halomonas sp. strain s2151	Acropora tissues	Gammaproteobacteria	644/667	96
	Shewanella piezotolerans	Montipora tissues	Gammaproteobacteria	676/698	96

^aAll enrichments were performed on MAMS medium (see Table 1).

^bSequences were aligned to the closest relative by using BLAST (1). The similarity was calculated without gaps taken into account.

^cThat is, the material(s) from which the bacteria were isolated.