. 2009 Mar 20;75(11):3469–3476. doi: 10.1128/AEM.02590-08

TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Description^a	Reference or source
Streptomyces strains
HK 803	Wild type; produces PLMs A through F	Riken, Japan
NP8	plmS₂-plmS₃ deletion mutant; Am^r; produces only PLM B	This work
NP8/pMSG1'	plmS₃ deletion mutant; Am^r Sp^r; produces only PLM G	This work
E. coli strains
TG2	supE hsdΔ5 thi Δ(lac-proAB) Δ(srl-recA)306::Tn10(Tet^r) F′[traD36 proAB⁺lacI^qlacZΔM15]	22
BW 25113/pIJ790	Recombination host; Chl^r	8
ET12567/pUZ8002	Nonmethylating strain harboring nontransmissible pUZ8002 (plasmid capable of mobilizing oriT-containing vectors)	8
BL21-Codon Plus(DE3)	E. coli expression host; Chl^r	Stratagene
Plasmids
pET15b	E. coli expression vector; Ap^r	Novagen
pMSG1	pGF200 with 0.7-kbp NdeI-BglI fragment replaced by 1.2-kbp NdeI-BamHI plmS2; Ap^r	7
pMSG1′	pMSG1 with aac(3)IV replaced by aadA; Sp^r	This work
pMSG4	pET15b with 921-bp NdeI-BamHI plmS₃ ORF; Ap^r	This work
pIJ773	Contains apramycin cassette for gene disruption; Ap^r Am^r	8
pIJ778	Contains streptomycin and spectinomycin cassette for gene disruption; Sp^r Am^r	8
Cosmid clone 3A11	Supercos 1 (Stratagene) derived; Ap^r Kan^r	21

Ap^r, ampicillin resistance; Am^r, apramycin resistance; Chl^r, chloramphenicol resistance; Kan^r, kanamycin resistance; Sp^r, spectinomycin resistance.