TABLE 1.
Organisms employed to asses the exclusivity of the invA qPCR TaqMan assay for Salmonella sp. detectiona
| Organism | No. of strains tested |
|---|---|
| Vibrio parahaemolyticus | 4b |
| Vibrio vulnificus | 1 |
| Escherichia coli | 9c |
| Enterobacter cloacae | 1 |
| Enterobacter aerogenes (ATCC 13048) | 1 |
| Cronobacter sakazakii (formerly E. sakazakii) | 1 |
| Yersinia enterocolitica | 1 |
| Yersinia pseudotuberculosis | 1 |
| Hafnia alvei | 2 |
| Morganella morganii | 1 |
| Edwardsiella tarda | 1 |
| Klebsiella pneumoniae | 1 |
| Proteus vulgaris | 1 |
| Pseudomonas aeruginosa | 1 |
| Serratia marcesans | 1 |
| Aeromonas hydrophila | 1 |
| Citrobacter freundii | 1 |
| Citrobacter koseri (ATCC 27028) | 1 |
| Staphylococcus aureus | 1 |
| Streptococcus faecalis | 1 |
| Bacillus subtilis | 1 |
| Bacillus cereus | 1 |
| Listeria monocytogenes | 1 |
| Listeria innocua | 1 |
| Shigella sonnei | 2 |
| Shigella flexneri | 2 |
| Shigella boydii | 2 |
| Shigella dysenteriae | 2 |
| Achromobacter spp. | 1 |
| Providencia stuartii (ATCC 33672) | 1 |
| Proteus mirabilis | 1 |
| Proteus hauseri (deposited as P. vulgaris) (ATCC 13315) | 1 |
| Total | 48 |
a
Exclusivity was demonstrated by the absence of positive signal for all strains tested; i.e., all invA qPCR results were negative.
b
V. parahaemolyticus pandemic strains VpHY145 and JYKVP6 and non-pandemic strains 428/00 and 357-99 (15).
c
Five E. coli classes (virotypes) that cause diarrheal diseases were included: strain 10009 (enterotoxigenic); strains 10010, 10015, 10016, 10017, and 10012 (enteroinvasive); strain 10023 (enterohemorrhagic); strain 10035 (enteropathogenic), and strain ATM395 (enteroaggregative).