TABLE 2.
Primers and probes used in this study
| Function and target | Primer or probe | Sequence (5′→3′)a | Position on geneb | Product size (bp) | Accession no.c | Reference or sourced |
|---|---|---|---|---|---|---|
| Generation of standards, invA | invA-T7-F2 | TAATACGACTCACTATAGGGAACAGTGCTCGTTTACG | 1 | 885 | DQ644631 | 10 |
| invA-T7-R1 | GCAGAGTTCCCATTGAAATGGTC | 865 | 10 | |||
| PCR | ||||||
| invA | invA_176F | CAACGTTTCCTGCGGTACTGT | 175 | 116 | DQ644631 | 4 |
| invA_291R | CCCGAACGTGGCGATAATT | 291 | 4 | |||
| invA_Tx_208 | TX-CTCTTTCGTCTGGCATTATCGATCAGTACCA-BHQ2 | 207 | 4 | |||
| myIC | dd-myIC-f | CTAACCTTCGTGATGAGCAATCG | 1 | 198 | FJ357008 | CFSAN/ORS |
| dd-myIC-r | GATCAGCTACGTGAGGTCCTAC | 198 | CFSAN/ORS | |||
| dd-myIC-Cy5 | Cy5-AGCTAGTCGATGCACTCCAGTCCTCCT-Iowa BlackRQ-Sp | 62 | CFSAN/ORS |
a
The sequence corresponding to the T7 promoter is underlined. TX, Texas Red.
b
Positions on genes are given according to the accession numbers.
c
Accession number at the NCBI (http://www.ncbi.nih.gov/).
d
ORS, Office of Regulatory Science.