TABLE 3.
Selected transcripts upregulated in cells grown on cDCE compared to cells grown on glycolate
| Sample | Locus taga | P valueb | Change (fold)c | Region or putative enzyme function |
|---|---|---|---|---|
| 1 | ctg94:3530095-ctg94:3530164 | 0.015 | 122.9 | Intergenic region |
| 2 | Bpro3336 | 0.005 | 111.0 | ABC transporter, extracellular ligand-binding receptor |
| 3 | Bpro0645d | 0.012 | 99.8 | GST-like protein |
| 4 | Bpro0646 | 0.012 | 87.5 | PNPox-related protein |
| 5 | Bpro3335 | 0.009 | 70.0 | ABC transporter, inner membrane translocator |
| 6 | Bpro0530 | 0.011 | 53.3 | HAD, type II |
| 7 | Bpro5186 | 0.013 | 51.5 | HAD, type II |
| 8 | Bpro0532 | 0.007 | 42.7 | Hypothetical protein |
| 9 | ctg94:3611882-ctg94:3611943 | 0.011 | 41.7 | Intergenic region |
| 10 | Bpro0531 | 0.015 | 40.9 | Sodium/solute symporter |
| 11 | Bpro5185 | 0.017 | 39.2 | Sodium/solute symporter |
| 12 | Bpro3334 | 0.010 | 30.8 | ABC transporter, inner membrane translocator |
| 13 | Bpro3333 | 0.013 | 27.1 | ABC transporter, ATPase component |
| 14 | Bpro0529 | 0.013 | 24.3 | Conserved hypothetical protein |
| 15 | ctg94:581640-ctg94:581753 | 0.033 | 21.7 | Intergenic region |
| 16 | ctg94:2876701-ctg94:2876845 | 0.011 | 20.8 | Intergenic region |
| 17 | ctg94:529265-ctg94:529416 | 0.014 | 20.0 | Intergenic region |
| 18 | Bpro5184 | 0.004 | 19.0 | Predicted membrane protein |
| 19 | Bpro3332 | 0.005 | 18.3 | ABC transporter, ATPase component |
| 20 | Bpro2731 | 0.016 | 17.9 | Conserved hypothetical protein |
| 23 | Bpro2396 | 0.017 | 14.8 | Heme peroxidase |
| 30 | Bpro0577 | 0.025 | 10.4 | CO DHase |
| 31 | Bpro5565 | 0.024 | 10.1 | CMO |
| 40 | Bpro5566 | 0.056 | 7.3 | Hlase |
| 44 | Bpro5564 | 0.010 | 6.3 | Alcohol dehydrogenase |
| 75 | Bpro3866 | 0.027 | 3.6 | Universal stress protein (UspA) |
| 81 | Bpro5301 | 0.010 | 3.5 | Cytochrome P450 |
| 83 | Bpro2732 | 0.018 | 3.4 | Transposase |
| 92 | Bpro4792 | 0.030 | 3.2 | Transposase |
| 95 | Bpro4575 | 0.024 | 3.1 | Transposase |
| 100 | Bpro3227 | 0.040 | 3.0 | Universal stress protein (UspA) |
Intergenic regions, which do not have locus tags, are indicated by their positions in the contig.
P values are based on a one-way analysis of variance Welch t test with Benjamini-Hochberg multiple-test correction and an FDR of 5% for the genes identified.
Genes upregulated at least 1.5-fold in cDCE-grown cells compared to glycolate-grown cells with an FDR of 5% (P < 0.05). The P value for Bpro5566 was 0.056.
Genes and the corresponding proteins that were identified as upregulated by growth on cDCE in microarray and proteomic experiments are indicated by bold type.