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. 2009 Mar 30;77(6):2320–2329. doi: 10.1128/IAI.01507-08

FIG. 1.

FIG. 1.

Expression profiles of ISL 929 and ISL 1373 in ticks and knockdown by RNAi. (A) Nymphal and adult I. scapularis ticks were allowed to feed on naïve mice, and tick salivary glands (SG) and midguts (MG) were harvested after 72 h of feeding. Expression of ISL 929 and ISL 1373 was assessed by RT-PCR as described in Materials and Methods and normalized to tick actin. U, unfed nymphs. (B) Adult female I. scapularis ticks were injected with 0.5 μl buffer alone or dsRNA corresponding to a shared coding region of ISL 929, ISL 1373, and Salp 13. RNAs extracted from salivary glands of control-injected (left) and dsRNA-injected (right) ticks were reverse transcribed and PCR amplified to assess the efficiency of gene knockdown by agarose gel electrophoresis of the PCR products using actin as a control. (C) Selected ticks from panel B were assessed by qPCR for the efficiency of knockdown. Significance compared to control-injected mice: *, P < 0.0001; **, P = 0.0012; Mann-Whitney U test.