TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence 5′→3′a |
|---|---|
| VPS21DISF | CCATCATTATTTCTACTATTTGTATTGCATTGTCGCATTCAGGATCAAAGAGCCAAAATACATACTAGCATGTGGAATTGTGAGCGGATA |
| VPS21DISR | ACCATGGAGATCTGTACACACTTGCCTATTTGTATATTACCTTAACTTCAGTCTCCTTGTTTATTTATATTTTCCCAGTCACGACGTT |
| VPS21AMPF | TCATCAGGATCCCCGAAGAAAAGGGGAAGGAGA |
| VPS21AMPR | TCATCAGGATCCGTAAAATTGCACGACTGTGCC |
| VPS21AMPRP | AAAACTGCAGGTAAAATTGCACGACTGTGCC |
| VPS21PBR | TTGAGTTAAAAACGCAGCTCC |
| VPS21SEQF | AATACTAGTAGTATCACCATC |
| VPS21SEQR2 | GGAGATCTGTACACACTTGCC |
| VPS21EAGMUTF | GAGCCAAAATACATACTAGCACGGCCGAGTCAACATCCAGCCCCCGC |
| VPS21EAGMUTR | GCGGGGGCTGGATGTTGACTCGGCCGTGCTAGTATGTATTTTGGCTC |
| YPT72DISF | AACGATACAGAGTTTAATTTAATTTAATTCAATTCAATTTAATTAATTAATCATATACACTTAATTTTCATGTGGAATTGTGAGCGGATA |
| YPT72DISR | AAACGAATTCGTGATATCTATTTGTTCTCTTTCTTCACCTGTGTATTTGAAATTGATTGTTATATTATTATTTCCCAGTCACGACGTT |
| YPT72AMPF3 | TCATCAGGATCCCCAAAAATATAGAAATGTTGCG |
| YPT72AMPR2 | TCATCAGGATCCATTAAAACTGGTAATGATTTGG |
| YPT72AMPRP | AAAACTGCAGATTAAAACTGGTAATGATTTGG |
| YPT72PBR | TTTACCAACACCAGAATCTCC |
| YPT72SEQF | GAAGATAATTAAAAGGCTGAC |
| YPT72SEQR | TTACTCCATCTTTGGCACTGG |
| YPT72SEQR2 | CAACAATTATAAACGAATTCG |
| YPT72XMAMUTF | TAATTAATCATATACACTTAATTTTCACCCGGGTCATCATCATCTAGAAAGAAAACA |
| YPT72XMAMUTR | TGTTTTCTTTCTAGATGATGATGACCCGGGTGAAAATTAAGTGTATATGATTAATTA |
| GFPXMAF | TCCCCCCGGGATGTCTAAAGGTGAAGAATTATTC |
| GFPXMAR | TCCCCCCGGGTTTGTACAATTCATCCATACC |
| GFPEAGF | TCACGGCCGATGTCTAAAGGTGAAGAATTATTC |
| GFPEAGR | TCACGGCCGTTTGTACAATTCATCCATACC |
| GFPSEQF | TACTTATCCACTCAATCTGCC |
| TUP1DISF | ATGTCCATGTATCCCCAACGCACCCAGCACCAACAACGTTTGACAGAGTTGTTGGATGCAATCAAAACTGTGTGGAATTGTGAGCGGATA |
| TUP1DISR | TTATTTTTTGGTCCATTTCCAAATTCTGGCTTTACAATCGCCACTACCTGTAGCGAAGATACCTTCGGTTGTTTTCCCAGTCACGACGTT |
| TUP1DETF | ATTTGAAAAAAGCGCACCCCC |
| TUP1DETF2 | GAACAAATCGCTTCCGGGTCC |
| TUP1DETR | GGTGCTGTGACAGAAGCAGAG |
| TUP1DETR2 | CCAAGAGTTTTCCGTCAGGGG |
| TUP1REGF | TCATCAGGATCCCTTCTTCCATCCCCACCAGCA |
| TUP1REGR | TCATCACTGCAGTTATACAACACATAGTATTCGAGG |
| ARG4DET2 | ATCAATTAACACAGAGATACC |
| HIS1F1268 | CCGCTACTGTCTCTACTTTG |
| URA3-5 | CCTATGAATCCACTATTGAACC |
| MET3DETF | CATCAAGTATACGTAATCTCC |
a
Engineered restriction enzyme sites are underlined.