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Binding of rIsdA to microtiter plate wells coated with loricrin (⧫), involucrin (▪), cytokeratin K10 (▴), or BSA (X). Increasing concentrations of rIsdA were incubated in wells for 1 h at room temperature. Bound protein was detected with mouse anti-IsdA antibodies and anti-mouse alkaline phosphatase-conjugated antibodies. Results are the means of triplicate wells from three independent experiments. OD405, optical density at 405 nm.
