FIG. 3.

Inhibition of rIsdA binding to immobilized ligands loricrin (A), involucrin (B), and cytokeratin (C). rIsdA was preincubated for 1 h at room temperature with increasing concentrations of loricrin (⧫), involucrin (▪), cytokeratin K10 (▴), or BSA (X) before incubation in protein-coated wells. Bound protein was detected with mouse anti-IsdA antibodies and anti-mouse alkaline phosphatase-conjugated antibodies. Values represent the means of triplicate wells taken from three independent experiments.