TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Staphylococcus aureus | ||
| SH1000 | Wild type | 25 |
| SRC005 | isdA::Tn917 Emr in SH1000 | 7 |
| SRC008 | isdAΔNEAT in SH1000 | 11 |
| SRC009 | isdAΔC in SH1000 | 11 |
| Escherichia coli | ||
| BL21(DE3) | F−ompT gal [dcm] [lon] hsdSB (rB− mB−) | Novagen |
| TOPP3 | Rifr [F′ proAB lacIqZΔM15 Tn10(Tetr)] Kanr | Stratagene |
| Lactococcus lactis | ||
| NZ9800 | Host for expression plasmids | 13 |
| Plasmids | ||
| pIsdA | IsdA overexpression vector | 7 |
| pMK4 | E. coli-S. aureus shuttle vector | 47 |
| pSRC001 | rIsdA complementation vector | 7 |
| pNZ8148 | L. lactis expression plasmid | 26 |
| pLIsdA | IsdA expression vector | 10 |
| pLΔNEAT | IsdAΔNEAT expression vector | 10 |
| pLΔC | IsdAΔC expression vector | 10 |
| pQE30 | Overexpression plasmid | Qiagen |
| pHuman K10 | pQE30 human cytokeratin 10 expression | 54 |
| pCMC-SPORT6-INV | Involucrin cDNA clone 4749952 | IMAGE consortium, MRC Geneservice |
| pQE30-INV | 6× His-tagged involucrin | This study |
| pET11a-LOR | Loricrin cDNA clone | Gift from D. Roop and P. Koch |