Skip to main content
. 2009 Mar 23;77(6):2427–2435. doi: 10.1128/IAI.00048-09

FIG. 5.

FIG. 5.

Strain-specific invasion inhibition of parasite growth using PfRh4 antibodies. (A) Parasite growth for the 3D7, W2mef, W2mefΔ175, and W2mefΔRh4 parasite lines grown in the presence of purified IgG from rabbit prebleed serum (NRS) or anti-Rh4 purified IgG antibodies (αRh4) in normal erythrocytes. (B) Growth of the 3D7 and W2mefΔ175 lines in neuraminidase-treated erythrocytes in the presence of purified IgG from rabbit preimmune serum, purified IgG from second-bleed serum, and purified IgG from third-bleed serum from rabbits immunized with PfRh4. (C) Growth of the 3D7 parasite line in the presence of a dilution series for purified nonspecific IgG from rabbit prebleed serum (NRS) and purified anti-Rh4 IgG antibodies (αRh4) in neuraminidase-treated erythrocytes shows that Rh4 antibody inhibition of parasite growth is concentration dependent. The final concentrations of IgG antibodies ranged from 0 to 2 mg/ml in each invasion assay. For all panels, parasite growth was measured as a percentage of the mean parasite growth for four wells, with nonspecific IgG from rabbit prebleed serum control added in each experiment. The error bars represent the standard error of the mean for duplicate wells in two independent experiments. The concentrations mentioned above refer to the total IgG purified using protein G columns.