FIG. 5.

Tn mutant Y1C12 cells exhibit increased sensitivity to phagocytosis and killing by PMNs. Phagocytosis and killing were determined 120 min after infection of human PMNs. PMNs were infected with wt, Y1C12, or cY1C12 C. canimorsus at an MOI of 1. Infections with nonopsonized (black), HI NHS-opsonized (gray), or C7-depleted NHS-opsonized bacteria (white) are shown. Results are the means of at least three independent experiments ± standard deviations (bars), including statistical significance between phagocytosis and killing of the wt and Y1C12 (unopsonized), with *** indicating a P value of <0.001 using a two-tailed, unpaired Student's t test.