FIG. 2.
Phylogenetic trees generated by the neighbor-joining method for traJ, traM, traS, traT, and traY sequences of several reference E. coli conjugative plasmids. Numbers at the branches are bootstrap proportions (displayed if >75) obtained from 100 replicates. Sequences were extracted from GenBank, except for plasmid pS88: pAPEC-O2-ColV (strain APEC O2; accession number AY545598), pAPEC-O1-ColBM (strain APEC O1; accession number DQ381420), plasmid F (accession number AP001918), p1658-97 (accession number AF550679), p53638 (accession number CP001064), pMAR7 (accession number DQ388534), pRK100 (accession number AY230886), pSMS-3-5 (accession number CP000971), pUTI89 (accession number CP000244), pVM01 (accession number EU330199), and plasmid R1 (accession number X13681) and its derepressed mutant pR1-19 (accession number M19710). Plasmid pS88 and the two APEC plasmids pAPEC-O2-ColV and pAPEC-O1-ColBM are highlighted in bold.