FIG. 6.

AnkA binds to ATC-rich sequences of the host chromatin. (A) AnkA-bound DNA was immunoprecipitated using an AnkA-specific MAb, cloned by LM-PCR, and sequenced. Sequences mT3-JHP and mT10-JHP were previously obtained in our laboratory (24). Infected cells were harvested at 72 h postinfection. (B) Binding of AnkA to the wild-type CYBB promoter probes A, B, and E, but not to mutated versions, was demonstrated by EMSA. The wild-type and mutated probes were incubated with rAnkA. Control reactions with no protein added were included as controls.