FIG. 1.

Binding of hnRNP A1 to the EV71 5′ UTR. (A) The binding of hnRNP A1 to the EV71 5′ UTR is specific. EMSA was performed as described in Materials and Methods. The EV71 5′ UTR was labeled with [³²P]GTP (1 ×10⁴ cpm per reaction) and incubated at 37°C for 30 min either alone or with 2 μg of hnRNP A1. A nonspecific 31-mer RNA oligonucleotide was end labeled with ³²PO₄ and reacted with 2 μg of hnRNP A1. (B) hnRNP A1 binds to the IRES of the EV71 5′ UTR. nt 1 to 90 and nt 91 to 745 were labeled with [³²P]GTP (1 ×10⁴ cpm per reaction), and EMSA was carried out as described above (A).