FIG. 2.

Identification of hnRNP A1 binding sites on the EV71 IRES. (A) The secondary structure of the 5′ UTR was predicted by MFold. (B) A serial deletion to eliminate each of the six stem-loops of the IRES was made. RNA probes were labeled with [γ-³²P]ATP (1 ×10⁴ cpm per reaction), and EMSA was carried out as described in the legend of Fig. 1A.