FIG. 3.

DN rab5 inhibits FMDV infection of IBRS-2 cells. (A and B) IBRS-2 cells were transfected to express wt rab5 (A) or DN rab5 (B) and infected with FMDV. Panels A and B show representative cells from one experiment. Cells expressing the rab protein (green) were identified by the EGFP tag. FMDV-infected cells (red) were identified as for Fig. 1. Yellow indicates rab-expressing cells that are infected. The cell nuclei are shown in blue. Bars, 10 μm. (C) Quantitative data for the experiments. Cells expressing either wt or DN rab5 were scored for infection. The data are shown as the percentage of the DN rab5-expressing cell population that was infected normalized to the level of infection of cells expressing the wt rab protein. The results are the means ± standard deviations for three independent experiments.