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. 2009 Apr 8;83(12):6247–6256. doi: 10.1128/JVI.02460-08

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Copyright © 2009, American Society for Microbiology

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FIG. 4. — Effect of DN rab5 on EE integrity and clathrin-mediated endocytosis. IBRS-2 cells were transfected to express wt rab5 (A and G) or DN rab5 (D and J). Cells expressing the rab protein (green) were identified by the EGFP tag. Panels B and E show the same cells as in panels A and D labeled for EE (EEA-1; red). Panels C and F show overlays of the panels immediately above. Areas of yellow indicate regions of colocalization. Panels H and K show internalization of Alexa-568-conjugated TN (red) for the same cells as shown in panels G and J, respectively. Panels I and L show overlays of the panels immediately above. The cell nuclei are shown in blue. Bars, 10 μm.