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. 2009 Apr 1;83(12):6171–6183. doi: 10.1128/JVI.02163-08

FIG. 1.

FIG. 1.

HSV is detectable in T cells from human herpes lesions. (A) Vesicle fluid collected from a herpetic lesion was stained and analyzed by flow cytometry for the presence of infected T cells using MAbs to CD3, CD4, and CD8 and rabbit polyclonal anti-HSV-2. Staining control was performed using rabbit Ig control instead of anti-HSV-2. The box in each dot plot contains the T cells positive for HSV antigens; numbers represent the percentage of T cells expressing HSV antigen. (B) A biopsy of a herpetic lesion from a second patient was stained with anti-CD3 and rabbit polyclonal anti-HSV-2 or rabbit Ig control. (C) Biopsies of a herpetic lesion (lesion skin) or control uninfected skin (normal skin) from a third patient were stained for the presence of infected T cells using anti-CD3 and rabbit polyclonal anti-HSV-2.