FIG. 6.

Detection of LFA-1 at the site of contact between HSV-infected fibroblasts and T cells. (A to C) PBMC stimulated with PHA (5 μg/ml for 2 days at 37°C) were coincubated for 1 h at 37°C with fibroblasts infected with HSV-1(F) at an MOI of 2 for 16 h, fixed, and stained for either active LFA-1 (red) and gD (green) (A and B) or total LFA-1 (red) and gD (green) (C). (D to F) PBMC stimulated with PHA as above were coincubated for 1 h at 37°C with fibroblasts infected with HSV-1(K26GFP) at an MOI of 2 for 16 h, fixed, and stained for either active LFA-1 (red) (D and E) or total LFA-1 (red) (F). K26GFP produces and incorporates into the virion the capsid protein VP26 fused to GFP (VP26-GFP). (G and H) PBMC stimulated with PHA as above were coincubated for 1 h at 37°C with uninfected fibroblasts, fixed, and stained for either active LFA-1 (red) (G) or total LFA-1 (red) (H). In all panels, the nuclei were stained with Hoechst.