FIG. 5.
Effects of residues 444, 446, and 482 on restoration of antibody neutralization potency. (A) Neutralizations with HCVpp bearing H77c, 02.E10, or single or triple reverse-mutated 02.E10 E2 were performed as described in the legend to Fig. 1. HCVpp were preincubated with CBH-2 or HC-11 at 37°C for 1 h at the concentrations specified on the y axis, prior to infection of Huh7.5 cells. Neutralization by each antibody is expressed on the z axis as percent reduction of luciferase activity relative to the level for a no-antibody control. On the x axis, the H77c, testing variant, and reverse-mutated variants are as indicated. (B) HMAb neutralization potency and binding affinity for H77c, 02.E10, and 02.E10 containing single or triple mutations. The table summarizes the IC50 of each HMAb for H77c and the mutated HCVpp and the Kd for binding of each HMAb to H77c and the mutated E2 proteins.