FIG. 5.

Inhibition of JNK1/2 and p38 MAPK phosphorylation decreases viral transcription and protein synthesis in the PCV2-infected PK15 cells. (A) Effects of JNK and p38 inhibitors on PCV2 spliced cap mRNA synthesis in infected cells. Total RNAs (1 μg) isolated from PCV2-infected cells at 24 h after treatment with JNK inhibitor 1 or SB202190 at the indicated concentrations were subjected to RT-PCR analysis of PCV2 spliced cap mRNA synthesis. The products were separated on a 1% agarose gel. β-Actin was used as an internal control. The results are representative of at least three independent experiments. (B) JNK inhibitor 1 and SB202190 reduce PCV2 protein expression. PCV2-infected PK15 cells at 72 h in the presence of the JNK inhibitor 1 (20 μM) or SB202190 (20 μM), as well as UV-irradiated PCV2-infected cells at 72 h, were assayed for the amount of PCV2 viral antigen by ICS. The amounts of PCV2 ORF2 protein expression are shown as percentages of PCV2-positive signals in cells infected with PCV2 alone. The data are mean values ± the SD based on three independent experiments; error bars show the SD.