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WNV C is sufficient to induce claudin degradation. (A) Total cell lysates from cell lines expressing Myc-tagged WNV C (clones 1 and 2) or the empty expression vector were analyzed by Western blotting for claudin-1, C, occludin, and β-actin expression. The arrow indicates the specific band for Cmyc. (B) cells (4 × 105) were plated on transwell filters and TER measured at 24 and 48 h postseeding. Values are the averages for three transwells for each cell line ± SD.
