TABLE 1.

Red recombination primers used in the study

Primer	Sequencea	Purpose
5′ gE-EnPas	GTTGGGCTCCCATTTTACCCGAAGATCGGCTGCTATCCCCGGGACCTGGATCGCGGGGCGGTGGTGGGGTTTCTTAGGATGACGACGATAAGTAGGG	gEctg (start codon point mutation)
3′ gE-EnPas	AACACAAACACCGAGAAGAAACCCCACCACCGCCCCGCGATCCAGGTCCCGGGGATAGCAGCCGATCTTCGGGTACAACCAATTAACCAATTCTGATTAG
5′ gD-366Del	GGCAGCCCTGGTCATTTGCGGAATTGTGTACTGGATGCGC_TAGATACCCCCCCTTAATGGAGGATGACGACGATAAGTAGGG	gDΔct (deletion from amino acid 366 but leaving stop codon)
3′ gD-366Del	CAGACCTGACCCCCCCGCACCCATTAAGGGGGGGTATCTA_GCGCATCCAGTACACAATTCCAACCAATTAACCAATTCTGATTAG
5′ gM-EnPas	GATACGCTCGACGTGTACTGTTCGCACTCGTCGTCCCCACTGGGACGCCCGGCCCCCAGAGGATGACGACGATAAGTAGGG	gMctg (start codon point mutation)
3′ gM-EnPas	GCGCGGAGTCGGGAGATCCTCTGGGGGCCGGGCGTCCCAGTGGGGACGACGAGTGCGAACCAACCAATTAACCAATTCTGATTAG
5′ UL20-EnPas	GCGACCCTTTGCGGTTTCGGTCTCCCCACCTCCACCGCACACCCCCTGACCCTGCGGGATGACCTTCCTCTGGTGGATCGAAGGATGACGACGATAAGTAGGG	UL20ctgctg (start codon and downstream site point mutations)
3′ UL20-EnPas	CTCGTCGACCAGATCTCGATCCACCAGAGGAAGGTCATCCCGCAGGGTCAGGGGGTGTGCGGTGGAGGTGGGGAGACCGAACAACCAATTAACCAATTCTGATTAG

^aThe regions homologous to HSV-1 DNA are in standard uppercase, the underlined sequences denote sites of mutation, the underscores represent deletion, and the regions which bind to the marker gene are in bold italics.