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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Arch Biochem Biophys. 2009 Jan 20;484(1):16–23. doi: 10.1016/j.abb.2009.01.011

Table 2.

Parameters from Linkage Analysis Obtained at pH 7.5, 37°C

Fit strategy of PEP response
Parameters from Equation 2 as in text (Figure 2) Vmax/Km (Supplemental Figure 10)
Ka-PEP (mM) 0.57±0.01 NA
Kix-FBP (mM) 0.00064±0.00005 0.00040±0.00006
Kix-ATP (mM) 0.81±0.06 1.5±0.3
Kix-Ala (mM) 2.3±0.2 2.5±0.3
Qax-FBP (ΔGax-FBP (kcal/mol)) 8.7±0.2 (-1.33±0.01) 18±1 (-1.78±0.03)
Qax-ATP (ΔGax-ATP (kcal/mol)) 0.16±0.01 (1.13±0.04) 0.09±0.04 (1.5±0.3)
Qax-Ala (ΔGax-Ala (kcal/mol)) 0.064±0.004 (1.69±0.04) 0.033±0.003 (2.10±0.06)