Expression of CreER and EGFP recapitulates the endogenous Gbx2
in Gbx2CreER/+ embryos. (A-C) Expression of
Gbx2 (A) and CreER (B) transcripts, and EGFP proteins (C) on
adjacent sagittal brain sections of Gbx2CreER/+
embryos at E12.5. The expression in the thalamus and the medial ganglionic
eminence is marked by the asterisk and arrowhead, respectively. The arrow
indicates thalamic axonal processes. (D-E″)
Double-immunofluorescence analysis of BrdU and GFP on coronal brain sections
of Gbx2CreER/+ embryos at
E12.5. The boxed area in D is magnified in E-E″. (F-G″)
Confocal images of immunofluorescence of pH3 and GFP on coronal brain sections
of Gbx2CreER/+ embryos at
E12.5. The boxed area in F is magnified in G-G″. Note that most of the
pH3-positive cells are negative for GFP, whereas two pH3-positive cells that
display weak GFP immunoreactivity are marked by the yellow arrowheads.
(H-I″) Confocal images of immunofluorescence of GFP and TuJ1 on
coronal brain sections of
Gbx2CreER/+ embryos at
E11.5. The boxed area in H is magnified in I-I″. ET, epithalamus; mb,
midbrain; Ncx, neocortex; PT, pretectum; PTh, prethalamus; TH, thalamus. Scale
bars: 1034 μm in A-C; 400 μm in D; 300 μm in F; 600 μm in H; 50
μm in E-E″,G-G″,I-I″.