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. 2009 Mar 20;136(8):1387–1396. doi: 10.1242/dev.028167

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Fig. 2. — Osr2-IresCre-mediated inactivation of Smo efficiently blocks Hedgehog signaling in the palatal mesenchyme by E13. (A) Whole-mount X-gal staining of an E11.5 Osr2-IresCre;R26R embryo showing specific Cre-mediated activation of lacZ expression in the primordia of the primary (arrowheads) and secondary (arrows) palate. Asterisks mark the sites of fusion between the medial nasal and the maxillary processes. (B) X-gal stained frontal section of E12.5 Osr2-IresCre;R26R embryo showing β-galactosidase activity throughout the palatal mesenchyme but absent in the palatal epithelium (arrowheads). (C,D) By E13.0, Ptch1 mRNA expression was dramatically downregulated in the palatal mesenchyme and persisted in the palatal epithelium (arrowhead) in the Osr2-IresCre;Smo^c/c embryo (D) in comparison with control littermate (C). (E,F) Gli1 mRNA expression was also dramatically downregulated in the palatal mesenchyme and persisted in the palatal epithelium (arrowhead) in the Osr2-IresCre;Smo^c/c mutant embryo (F) in comparison with the control littermate (E) by E13.0. lnp, lateral nasal process; max, maxillary process; mnp, medial nasal process; p, palatal shelf; t, tongue. Scale bar: 100 μm.