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. 2009 Mar 20;136(8):1387–1396. doi: 10.1242/dev.028167

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Fig. 6. — Comparison of Ccnd1 protein and Ccnd2 mRNA expression in the developing palate in control and Osr2-IresCre;Smo^c/c mutant embryos at E13.5. (A,B) Ccnd1 immunostaining (shown in brown) in frontal sections of control (A) and Osr2-IresCre;Smo^c/c mutant (B) embryos. Ccnd1 expression in the tooth bud epithelium (arrows) served as internal controls because Osr2-IresCre was not expressed in this tissue. Arrowheads point to the palatal epithelium, which appeared thinner in the Osr2-IresCre;Smo^c/c mutant embryos than in the control littermates. (C,D) In situ hybridization detection of Ccnd2 mRNA (in blue) in frontal sections of control (C) and Osr2-IresCre;Smo^c/c mutant (D) embryos. p, palatal shelf.