Fig. 10.

pdr10∆ cells accumulate excess Pdr12 in the plasma membrane. a Total membranes were prepared from log phase cultures of strains W303-1A (wild type) and NRY243 (pdr10∆). The indicated proteins were detected by immunoblotting, with equal protein loaded for each strain. b Strains NRY915 (wild type) and NRY925 (pdr10∆) were grown to log phase in YPD, and Pdr12-GFP was examined by fluorescence microscopy. A collage of representative images is shown. Pdr12-GFP function was confirmed by sorbate sensitivity. Bar, 6 μm