Fig. 12.

Pdr5 and phosphosphingolipids are required for pdr10∆ phenotypes. a Serial dilutions of overnight cultures of strains W303-1A (wild type), NRY243 (pdr10∆), NRY201 (pdr5∆), NRY230 (pdr5∆ pdr10∆), NRY212 (pdr15∆), NRY236 (pdr10∆ pdr15∆), NRY410 (ipt1∆), NRY534 (pdr10∆ ipt1∆), NRY409 (sur1∆), and NRY526 (pdr10∆ sur1∆) were spotted onto YPD plates containing Calcofluor White (CW; left column) or sorbate buffered with sodium succinate (right column). b Strains W303-1A, NRY243, NRY409, and NRY526 were assayed for PE accessibility to TNBS labeling after overnight labeling with ³²P-phosphate as described under Materials and Methods. c Total Pdr12 and Chs3 levels were examined by immunoblotting equal amounts of total membrane proteins isolated from log phase cultures of strains W303-1A, NRY230, and NRY526. d Strains W303-1A, NRY243, NRY409, NRY526, NRY410, and NRY534 were grown to log phase, stained for chitin, and examined by fluorescence microscopy. Bar, 5 μm