Fig. 2.

Pdr10 is required for normal response to Calcofluor and sorbate. a Spot assays were performed using serial dilutions prepared from overnight cultures of strains W303-1A (wild type), NRY243 (pdr10∆), NRY201 (pdr5∆), and NRY230 (pdr5∆ pdr10∆). All plates were grown for 2 days at 30°C unless otherwise noted. YPD plates contained either 2 μM ketoconazole (keto), 20 μM rhodamine 6G (Rh. 6G), or 100 ng/ml cycloheximide (CHX). b Halo assays were performed on YPD plates with various detergents. Aliquots from overnight cultures were diluted into YPD top agar as described under Materials and Methods. Top row, strains W303-1A, NRY243, and NRY601 (pdr10∆ chs3∆) were assayed with dodecyl maltoside (DDM). Middle row, the same strains were assayed with dodecyl phosphocholine (DPC). Bottom row, strains YPH499 (wild type) and YHW10 N (pdr10∆) were assayed with DDM. c Spot assays were performed as in A using strains W303-1A, NRY243, and NRY602 (chs3∆), NRY601 and NRY608 (chs6∆), and NRY610 (pdr10∆ chs6∆) on YPD plates (top) or YPD plates containing 6 μg/ml Calcofluor White (CW; middle) or 100 μg/ml Congo Red (CR; bottom). d Similar assays were performed using strains W303-1A (wild type), NRY243 (pdr10∆), NRY301 (pdr12∆), and NRY366 (pdr10∆ pdr12∆) on YPD plates buffered to pH 3.5 with 50 mM sodium succinate with or without 4 mM potassium sorbate