Fig. 6.

Increased phosphatidylethanolamine is accessible to TNBS labeling in pdr10∆ cells. a Strains W303-1A and NRY243 were grown to log phase, incubated with or without α-factor, and then stained with filipin to visualize sterols by fluorescence microscopy. Bar, 5 μm. b Exposure of endogenous aminophospholipids to TNBS was assayed with cells grown overnight in the presence of ³²P-phosphate as described under Materials and Methods. Strains used were W303-1A (wild type), NRY243 (pdr10∆), NRY549 (lem3∆), NRY551 (pdr10∆ lem3∆), NRY427 (dnf1∆ dnf2∆), and NRY558 (pdr10∆ dnf1∆ dnf2∆)