Fig. 9.

Loss of Dnf1 and Dnf2 function in pdr10∆ cells is associated with their increased chitin deposition. a Serial dilutions from overnight cultures of strains W303-1A (wild type), NRY243 (pdr10∆), NRY427 (dnf1∆ dnf2∆), NRY558 (pdr10∆ dnf1∆ dnf2∆), NRY549 (lem3∆), and NRY551 (pdr10∆ lem3∆) were spotted onto YPD plates at increasing concentrations of Calcofluor White (CW). b Log phase cultures of strains W303-1A, NRY243, NRY427, and NRY558 were stained for chitin and examined by fluorescence microscopy. Bar, 5 μm. c Serial dilutions of strains W303-1A, NRY243, NRY549, and NRY551 were spotted onto YPD plates buffered to pH 3.5 with sodium succinate and containing potassium sorbate. d Serial dilutions of strains W303-1A, NRY243, NRY301 (pdr12∆), and NRY366 (pdr10∆ pdr12∆) were spotted onto YPD plates containing Calcofluor White