Table 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Source/reference |
|---|---|---|
| W303-1A | MATaura3-1 leu2-3,112 his3-11,15 trp1-1 ade2-1 can1-100 | Sherman et al. (1986) |
| W303-1B | MATα ura3-1 leu2-3,112 his3-11,15 trp1-1 ade2-1 can1-100 | Sherman et al. (1986) |
| BYB67 | MATa/MATα ura3-1/ura3-1 leu2-3,112/leu2-3, 112 his3-11,15/his3-11,15 trp1-1/trp1-1 ADE2/ade2-1 LYS2/lys2::hisG can1-100/can1-100 | Shulewitz et al. (1999) |
| WKK7 | W303-1A ste6∆::HIS3 | Kuchler et al. (1989) |
| NRY201 | W303-1A pdr5∆::HIS3MX6 | Schüller et al. (2007) |
| NRY212 | W303-1A pdr15∆::TRP1 | Schüller et al. (2007) |
| NRY230a | W303-1A pdr5∆::URA3MX4 pdr10∆::HIS3MX6 | This study |
| NRY236a | W303-1A pdr10∆::HIS3MX6 pdr15∆::TRP1 | This study |
| NRY243b | W303-1A pdr10∆::HIS3MX6 | This study |
| NRY251b | W303-1A pdr10∆::URA3MX4 | This study |
| NRY301b | W303-1A pdr12∆::HIS3MX6 | This study |
| NRY334b | W303-1A YOL075c∆::URA3MX4 | This study |
| NRY336b | W303-1A pdr18∆::HIS3MX6 | This study |
| NRY366a | NRY251 pdr12∆::HIS3MX6 | This study |
| NRY407b | W303-1B cho1∆::HIS3MX6 | This study |
| NRY427a | W303-1A dnf1∆::URA3MX4 dnf2∆::kanMX4 | This study |
| NRY409b | W303-1A sur1∆::URA3MX4 | This study |
| NRY410b | W303-1A ipt1∆::URA3MX4 | This study |
| NRY526a | NRY243 sur1∆::URA3MX4 | This study |
| NRY534a | NRY243 ipt1∆::URA3MX4 | This study |
| NRY549b | W303-1A lem3∆::URA3MX4 | This study |
| NRY551a | W303-1A pdr10∆::HIS3MX6 lem3∆::URA3MX4 | This study |
| NRY558a | W303-1A pdr10∆::HIS3MX6 dnf1∆::URA3MX4 dnf2∆::kanMX4 | This study |
| NRY601b | NRY243 chs3∆::URA3MX4 | This study |
| NRY602b | W303-1A chs3∆::URA3MX4 | This study |
| NRY608b | W303-1A chs6∆::URA3MX4 | This study |
| NRY610b | NRY243 chs6∆::URA3MX4 | This study |
| NRY802c | W303-1A ADE2 lys2::hisG | This study |
| NRY804c | W303-1B ADE2 lys2::hisG | This study |
| NRY904d | W303-1A LEM3-mh::kanMX6 | This study |
| NRY906d | NRY804 PDR5-GFP::HIS3MX6 | This study |
| NRY908d | W303-1A pdr10∆::HIS3MX6 LEM3-mh::kanMX6 | This study |
| NRY912d | NRY802 PDR10-GFP::HIS3MX6 | This study |
| NRY914d | NRY802 LEM3-GFP::HIS3MX6 | This study |
| NRY915d | NRY804 PDR12-GFP::kanMX6 | This study |
| NRY916d | NRY802 pdr10∆::URA3MX4 PDR5-GFP::HIS3MX6 | This study |
| NRY918d | NRY802 pdr10∆::URA3MX4 LEM3-GFP::HIS3MX6 | This study |
| NRY921d | NRY802 DNF1-GFP::HIS3MX6 | This study |
| NRY923d | NRY802 DNF2-GFP::HIS3MX6 | This study |
| NRY925d | NRY802 pdr10∆::HIS3MX6 PDR12-GFP::kanMX6 | This study |
| NRY929d | NRY802 pdr10∆::URA3MX4 DNF2-GFP::HIS3MX6 | This study |
| NRY930d | W303-1A PDR10-mh::kanMX6 | This study |
| NRY932d | NRY802 pdr10∆::URA3MX4 DNF1-GFP::HIS3MX6 LYS2 | This study |
| YPH499 | MATaade2-101ochis3-∆200 leu2-∆1 lys2-801a trp1-∆63 ura3-52 | Sikorski and Hieter (1989) |
| YHW10 N | YPH499 pdr10∆::URA3 | This study |
aConstructed by sporulation and tetrad dissection of appropriate diploid parents
bConstructed by integration of PCR products amplified with appropriate primers using pFA6aHIS3MX6 or pAG60 as template and verified by PCR on genomic DNA
cConstructed by tetrad dissection of BYB67
dConstructed by integration of PCR products amplified with appropriate primers using pFA6a-GFP(S65T)-HIS3MX6, pFA6a-GFP(S65T)-kanMX6, or pNR99A as template and verified by PCR on genomic DNA