Table 3.

Association of membrane proteins with the detergent-resistant membrane fraction^a

Protein	Wild type		pdr10∆
	% DRM	% Soluble	% DRM	% Soluble
Pdr12	33 ± 11	54 ± 10	61 ± 6	28 ± 11
Chs3	17 ± 9	62 ± 6	19 ± 9	56 ± 10
Lem3-mh	19 ± 5	75 ± 7	24 ± 4	72 ± 1
Gas1	47 ± 5	37 ± 1	45 ± 2	36 ± 2
Pdr5	57 ± 11	23 ± 10	69 ± 9	14 ± 8

Note: Membranes were prepared and analyzed by cold Triton X-100 extraction, Optiprep density gradient flotation, and quantitative immunoblotting as described under Materials and Methods. For Pdr12 and Chs3, results are based on four independent experiments. All other proteins were examined at least twice. Strains were W303-1A or NRY904 (wild type with or without chromosomally mh-tagged Lem3) and NRY243 or NRY908 (pdr10∆ with or without chromosomally mh-tagged Lem3)