Fig. 5.

Herbivore-induced transcription of an A. thaliana (E)-β-caryophyllene synthase gene homologue in A. lyrata ssp. petraea rosette leaves. Semi-quantitative RT-PCR analysis of transcript levels of an A. lyrata ssp. petraea gene (AlCarS) with high sequence similarity to the A. thaliana (E)-β-caryophyllene synthase (At5g23960/AtTPS21). Induced transcripts were observed only in rosette leaves of plants that emitted (E)-β-caryophyllene after 20 h of feeding by P. xylostella. Control reactions were performed with RNA from undamaged clonal plants of the same line. Reactions with primers for actin-8 were performed to judge equality of the cDNA template concentration