Table 1.
Temperature (°C) | Genotype | Cortical (%)* | Gastrulation (%)† | Hatch rate (%) |
---|---|---|---|---|
25 | Wild type | 92 | 91 | 89 |
nopoSZ3004 | 62 | 65 | 12 | |
nopoSZ3004/Df(2R)Exel7153 | 67 | 12 | 0 | |
nopoZ1447 | 72 | 0 | 0 | |
nopoZ1447/Df(2R)Exel7153 | 68 | 0 | 0 | |
pCaSpeR4-CG5140/+; nopoZ1447 | 95 | 92 | 87 | |
nopoExc142 | 15 | 0 | 0 | |
nopoExc142/Df(2R)Exel7153 | 7 | 0 | 0 | |
pCaSpeR4-CG5140/+; nopoExc142 | 93 | 90 | 83 | |
18 | Wild type | 97 | 97 | 89 |
nopoSZ3004 | 78 | 86 | 76 | |
nopoZ1447 | 67 | 1 | 0 |
Embryos collected from females of the indicated genotypes were fixed for DNA and tubulin staining (see Materials and methods for details).
Percentage of embryos that develop to cycle 10 or beyond. For each genotype, at least 200 embryos (2-2.5 hours) were scored.
Percentage of embryos that develop to the initiation of gastrulation or beyond. For each genotype, at least 200 embryos (3-4 hours) were scored.