Table 2.

The nopo phenotype is suppressed by mnk

		Mitotic spindle defects (% spindles)†
		Bipolar spindles
		Abnormal centrosome number			Tripolar spindles	Gastrulation (% embryos)¶	Hatch rate (%)
Genotype	MI*	Decreased‡	Increased§	Barrel-shaped
Wild type	0.63	<1	0	<1	<1	100	89
nopoZ1447	0.95	62	13	64	10	0	0
mnk6006	0.64	1	<1	1	<1	99	80
mnk6006 nopoZ1447	0.68	<1	<1	<1	<1	77	0

Embryos collected from females of the indicated genotypes were used to determine hatch rates or were fixed for DNA and tubulin staining (see Materials and methods for details).

^*Mitotic index (MI)=% embryos in mitosis/total number of embryos. More than 300 embryos were scored per genotype. Chromosome condensation and the presence of a mitotic spindle were used as the criteria for mitosis.

^†All mitotic spindles (>500 total) in a single focal plane were scored using at least 25 embryos per genotype.

^‡Spindles with centrosomal detachment at one or both poles.

^§Spindles with >1 centrosome per pole (one or both poles). Telophase spindles were not scored because centrosome duplication occurs during this phase in the early embryo.

^¶Stained embryos (3-4 hours) were scored for development to the initiation of gastrulation (or beyond). More than 200 embryos were scored per genotype.