Table 3.
Quantification of defects in ben-derived embryos
|
Number of nuclei/embryo (% embryos)
|
nopo-like spindles (% embryos)‡ | |||||
|---|---|---|---|---|---|---|
| Genotype | MI* | Hatch rate (%) | 1 | 2-8 | >8 | |
| Wild type | 0.63 | 89 | − | − | − | <1 |
| ben1 | 0.92 | 2† | 72 | 12 | 16 | 80 |
| ben1/Df(1)KA10 | 0.80 | 0 | 40 | 43 | 17 | 50 |
Embryos collected from females of the indicated genotypes were used to determine hatch rates or were fixed for DNA and tubulin staining (see Materials and methods for details). At least 50 stained embryos were analyzed per genotype. Eggs/embryos in which DNA and tubulin were not visualized by staining were excluded from analysis.
Mitotic index (MI) = % embryos in mitosis/total number of embryos. Chromosome condensation and the presence of a mitotic spindle were used as the criteria for mitosis.
For ben1 homozygotes, only 100 embryos were scored for hatching owing to low egg-laying rates.
Embryos in which the majority of mitotic spindles were barrel-shaped and/or lacking centrosomes were scored as being `nopo-like'.