Fig. 6.

Western bolt analysis for MPAK and PI3K/AKT pathways in [6]-gingerol-treated pancreatic cancer cells. The BxPC-3 (A) and HPAC (B) were incubated with 400 µM of gingerol for indicated times. Cells were lysed and the cell lysates (30 µg) were resolved by SDS-PAGE. AKT, ERK and JNK activation was analyzed by Western blotting with anti-phospho-AKT, anti-phosph-ERK, anti-phosph-JNK or anti-p85-specific antibodies as indicated. Equal protein loading was confirmed by probing the membranes with antibodies detecting the respective unphosphorylated proteins (ERK and AKT). Three replicate experiments were done with similar results.