Table 1.
Genes induced by U-STAT1
| Gene symbol | PROBE_ID | BJ |
BJ |
hTERT-HME |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | YF | IFN-β |
IFN-γ |
IFN-β |
IFN-γ |
||||||
| 6 h | 48 h | 6 h | 48 h | 6 h | 48 h | 6 h | 48 h | ||||
| IFI27* | ILMN_1661581 | 4.3 | 8.1 | 6.1 | 46 | 1.9 | 15 | (2.1) | (51) | 0.0 | (1.0)** |
| BST2 | ILMN_1723480 | 3.7 | 8.1 | 12 | 22 | 5.5 | 25 | 18 | 150 | 24 | 33 |
| OAS1* | ILMN_1658247 | 3.1 | 6.3 | 10 | 7.9 | 2.4 | 2.3 | (26) | (31) | (1.5) | (1.0)** |
| ILMN_1675640 | 5.1 | 6.9 | 35 | 22 | 4.6 | 4.6 | (22) | (25) | 0.0 | 0.0 | |
| OAS2* | ILMN_1674063 | 4.1 | 6.2 | 9.8 | 11 | 4.5 | 4.0 | (11) | (23) | (2.3) | (1.0)** |
| OAS3 | ILMN_1745397 | 2.5 | 2.6 | 6.3 | 7.7 | 2.5 | 2.7 | 8.4 | 13 | 3.7 | 2.8 |
| STAT1 | ILMN_1690105 | 2.2 | 2.8 | 3.2 | 2.8 | 4.0 | 4.3 | 6.8 | 7.9 | 16 | 7.2 |
| ILMN_1777325 | 1.7 | 2.0 | 2.0 | 2.0 | 2.4 | 2.8 | 4.9 | 5.1 | 9.5 | 3.9 | |
| IFI44 | ILMN_1760062 | 2.6 | 3.3 | 6.2 | 4.1 | 2.6 | 2.1 | 130 | 180 | 9.5 | 8.8 |
| IFI44L | ILMN_1723912 | 5.2 | 7.7 | 13 | 13 | 5.5 | 6.3 | 43 | 73 | 3.6 | 2.4 |
| IFIH1 | ILMN_1781373 | 2.7 | 4.0 | 12.8 | 3.9 | 5.3 | 2.5 | 28 | 27 | 22 | 3.9 |
| IFITM1 | ILMN_1801246 | 2.0 | 2.5 | 2.1 | 3.4 | 1.7 | 2.9 | 22 | 76 | 6.4 | 4.7 |
| IFI35 | ILMN_1745374 | 2.1 | 2.2 | 3.9 | 3.0 | 3.4 | 3.3 | 3.7 | 5.2 | 9.4 | 3.6 |
| IFIT3* | ILMN_1701789 | 2.9 | 2.4 | 5.8 | 3.0 | 4.8 | 3.0 | (12) | (3.7) | (15) | (1.0)** |
| MX1* | ILMN_1662358 | 3.6 | 5.3 | 6.7 | 5.9 | 2.5 | 1.7 | (53) | (85) | (1.0)** | 0.0 |
| IRF7 | ILMN_1798181 | 3.0 | 4.3 | 6.1 | 3.3 | 1.6 | 1.2 | 12 | 11 | 2.4 | 1.6 |
| G1P2 | ILMN_1813289 | 2.7 | 3.0 | 3.6 | 4.2 | 1.5 | 1.6 | 27 | 41 | 5.0 | 2.7 |
| IFIT1* | ILMN_1707695 | 2.3 | 2.9 | 5.1 | 2.9 | 1.4 | 0.8 | (45) | (21) | (1.0)** | 0.0 |
| PLSCR1 | ILMN_1752889 | 2.4 | 3.2 | 6.3 | 3.2 | 3.1 | 1.8 | 4.8 | 4.7 | 2.4 | 1.0 |
| HERC6 | ILMN_1654639 | 3.4 | 3.7 | 5.4 | 6.3 | 1.7 | 2.4 | 35 | 100 | 9.9 | 7.8 |
| FLJ20035 | ILMN_1795181 | 2.1 | 2.9 | 3.5 | 4.0 | 3.0 | 3.4 | 32 | 80 | 22 | 17 |
| EPSTI1 | ILMN_1688566 | 2.5 | 3.4 | 4.2 | 3.4 | 3.7 | 4.0 | 7.3 | 11 | 12 | 4.9 |
The expressions of 22,184 probes (a gene is represented by 1–3 probes) in an Illumina Sentrix HumanRef-8 Expression Bead Chip were analyzed by using mRNA from BJ cells transfected with empty vector (Vec), wild-type STAT1 (WT), or Y701F-STAT1 (YF). BJ cells were treated with IFN-β (3 units/mL) or IFN-γ (0.3 ng/mL) for 6 or 48 h; hTERT-HME1 cells were treated with IFN-β (5 units/mL) or IFN-γ (0.1 ng/mL) for 6 or 48 h. The numbers in the table are fold inductions compared with the signals from the mRNAs of control cells (empty vector-transfected BJ cells for WT and YF, untreated BJ or hTERT-HME1 cells for IFN-treated cells). The average signal for each probe was used to determine expression levels. The genes with average signals below 25 in the control and the treated cells, or with detection P values more than 0.05 in the treated cells compared with the control, were excluded from the analyses.
*IFI27, OAS1, OAS2, IFIT3, Mx1, and IFIT1 were not detected in untreated control hTERT-HME1 cells but were induced in response to IFN. In these cases, the numbers in parentheses are fold inductions compared with the levels of expression in cells treated with IFN-γ for 6 or 48 h (denoted by double asterisks in the last column) instead of untreated cells. All of the genes in this table except HERC6, FLI20035, and EPSTI1 are known to play roles related to immune responses.