Table 1.

Dipping of Crocus wheat at various stages of microspore development

Stage of dippinga	Plasmid	Number of florets dipped	Agro strain	Number of T1 seeds	Southern confirmation	Lines generated
Mid-late uninucleate	pDs(Hyg)35S	168	C58C1	10	T4, T5, T6	3B2
Early uninucleate	pDs(Hyg)35S	336	C58C1	1	T2, T5	14C1
Late uninucleate	pBECKSred	174	AGL1	33	T1, T2, T3	1CR1
Bi-nucleateb	pBECKSred	5,000	AGL1	3,000	0 (red foci on some endosperm)	0
Tri-nucleatec	pBECKSred	5,600	AGL1	4,200	0	0

The stage of dipping, plasmids used, the number of florets dipped, the Agrobacterium strains, the number of T1 seeds, the generation in which T-DNA integration was confirmed and the designation of each transformant are given

^aThe stages of microspore development at spike dipping. The uninucleate microspore stage occurs over the 7 days when the spike is still in the sheath and has not emerged

^bThe bi-nucleate stage occurs as the spike is fully emerged from the sheath

^cThe tri-nucleate stage occurs 2 days prior to anthesis (Xiang-Yuan and De-Cai 1983)