Table 5. Infrastructure requirements of commercial versus rtLC DBS assays for viral load determination.
Infrastructure | Commercial assay | rtLC DBS assay |
Bio-safety cabinet | 1 | 1 |
Extraction Hood | 1 | 1 |
Refrigerated Centrifuge | 1 | 1 |
PCR Hood | 2 | 1 |
Thermocycler (LightCycler) | 1 | 1 |
Freezer Storage (in Celsius) | −70 | −20 |
Lab space (dedicated areas) | 5 | 3 |
Plate washer | Yes | No |
Incubator | Yes | No |
Plate Optical Density reader | Yes | No |