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. Author manuscript; available in PMC: 2009 May 29.
Published in final edited form as: Channels (Austin). 2008 Nov 27;2(6):413–418. doi: 10.4161/chan.2.6.6874

Figure 1.

Figure 1

Screen for Activatory Mutations in KcsA. (A) Wild-type KcsA does not complement the growth of K+-auxotrophic E. coli (TK2420) on low [K+] media (7.5 mM KCl). As a positive control KirBac2.1 complements growth well. The vector is pQE60-Lac. (B) Overall scheme for the random mutagenesis protocol.